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Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.

CRF Polyclonal Antibody

BS-0246R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetCRH
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CRF Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1392
  • Target name
    CRH
  • Target description
    corticotropin releasing hormone
  • Target synonyms
    corticoliberin; corticotropin-releasing factor; CRF; CRH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06850
  • Protein Name
    Corticoliberin
  • Reactivity
    Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The GABAA Receptor alpha2 Subunit Activates a Neuronal TLR4 Signal in the Ventral Tegmental Area that Regulates Alcohol and Nicotine Abuse. Balan I et al., 2018 Apr 21, Brain Sci
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  • Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity. Balan I et al., 2018 Mar, Brain Behav Immun
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