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ProductsBack/CRF Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, DAB staining.

CRF Polyclonal Antibody

BS-0246R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetCRH
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Overview

  • Supplier
    Bioss
  • Product Name
    CRF Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:500-2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1392
  • Target name
    CRH
  • Target description
    corticotropin releasing hormone
  • Target synonyms
    CRF, CRH1, corticoliberin, corticotropin-releasing factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06850
  • Protein Name
    Corticoliberin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Balan I, Warnock KT, Puche A, et al. The GABA(A) Receptor α2 Subunit Activates a Neuronal TLR4 Signal in the Ventral Tegmental Area that Regulates Alcohol and Nicotine Abuse. Brain Sci. 2018,8(4). doi: 10.3390/brainsci8040072
    Read this paper
  • Balan I, Warnock KT, Puche A, et al. Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity. Brain Behav Immun. 2018,69:139-153. doi: 10.1016/j.bbi.2017.11.008
    Read this paper

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Figure 1. Western blot analysis of CRF/CRH using anti-CRF/CRH antibody (A00629-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRF/CRH antigen affinity purified polyclonal antibody (A00629-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRF/CRH at approximately 25 kDa. The expected band size for CRF/CRH is at 21 kDa.
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