More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CrkL antibody

CrkL antibody

GTX107677
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCRKL
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    CrkL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.33 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1399
  • Target name
    CRKL
  • Target description
    CRK like proto-oncogene, adaptor protein
  • Target synonyms
    crk-like protein; v-crk avian sarcoma virus CT10 oncogene homolog-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46109
  • Protein Name
    Crk-like protein
  • Scientific Description
    This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • miR-124-3p Suppresses the Invasiveness and Metastasis of Hepatocarcinoma Cells via Targeting CRKL. Majid A et al., 2020, Front Mol Biosci
    Read more
  • miR-429-CRKL axis regulates clear cell renal cell carcinoma malignant progression through SOS1/MEK/ERK/MMP2/MMP9 pathway. Wang J et al., 2020 Jul, Biomed Pharmacother
    Read more
  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more
  • CRKL oncogene is downregulated by p53 through miR-200s. Tamura M et al., 2015 Aug, Cancer Sci
    Read more

Related products

Product group Antibodies
Everest Biotech
Goat anti-CRKLEB05254
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCRKL
  • SizePrice
Product group Antibodies
Biomatik
CRKL Polyclonal AntibodyCAC14637
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetCRKL
  • SizePrice
Product group Antibodies
Assay Genie
CRKL AntibodyCAB0511
TargetCRKL
  • SizePrice
Product group Antibodies
Bioss Antibodies
Phospho-Crkl (Tyr207) Recombinant Antibody, AbBy Fluor-594 ConjugatedBSM-61642R-BF594
ApplicationsWestern Blot
TargetCRKL
  • SizePrice
Figure 1. Western blot analysis of CRKL using anti-CRKL antibody (A02100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (Catalog # A02100-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRKL at approximately 35 kDa. The expected band size for CRKL is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-CRKL Antibody Picoband(r)A02100-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
TargetCRKL
  • SizePrice
Anti-CrkL Antibody
Product group Antibodies
Antibodies.com
Anti-CrkL AntibodyA95375
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CRKL Antibody144-11735
ApplicationsWestern Blot
TargetCRKL
  • SizePrice
Product group Antibodies
GeneTex
CrkL (phospho Tyr207) antibodyGTX133949
ApplicationsWestern Blot
TargetCRKL
  • SizePrice
WB analysis of various sample lysates using GTX32539 CrkL antibody. Dilution : 1:500 Loading : 25microg per lane
Product group Antibodies
GeneTex
CrkL antibodyGTX32539
ApplicationsWestern Blot
TargetCRKL
  • SizePrice
Product group Antibodies
GeneTex
CrkL (phospho Tyr207) antibodyGTX79096
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCRKL
  • SizePrice