Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CrkL antibody
GTX107677
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCRKL
Overview
- SupplierGeneTex
- Product NameCrkL antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.33 mg/ml
- ConjugateUnconjugated
- Gene ID1399
- Target nameCRKL
- Target descriptionCRK like proto-oncogene, adaptor protein
- Target synonymscrk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
- HostRabbit
- IsotypeIgG
- Protein IDP46109
- Protein NameCrk-like protein
- Scientific DescriptionThis gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Majid A, Wang J, Nawaz M, et al. miR-124-3p Suppresses the Invasiveness and Metastasis of Hepatocarcinoma Cells via Targeting CRKL. Front Mol Biosci. 2020,7:223. doi: 10.3389/fmolb.2020.00223Read this paper
- Wang J, Wang C, Li Q, et al. miR-429-CRKL axis regulates clear cell renal cell carcinoma malignant progression through SOS1/MEK/ERK/MMP2/MMP9 pathway. Biomed Pharmacother. 2020,127:110215. doi: 10.1016/j.biopha.2020.110215Read this paper
- Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8Read this paper
- Tamura M, Sasaki Y, Kobashi K, et al. CRKL oncogene is downregulated by p53 through miR-200s. Cancer Sci. 2015,106(8):1033-40. doi: 10.1111/cas.12713Read this paper
Datasheet
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