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CRM1/XPO1 Antibody

CAB0299
Assay Genie
TargetXPO1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    CRM1/XPO1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7514
  • Target name
    XPO1
  • Target description
    exportin 1
  • Target synonyms
    CRM-1, CRM1, emb, exp1, exportin-1, chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, exportin 1 (CRM1 homolog, yeast), exportin-1 (required for chromosome region maintenance)
  • Host
    Rabbit
  • Protein IDO14980
  • Protein Name
    Exportin-1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate All lanes: XPO1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa
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Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: rat testis tissue lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse testis tissue lysates, Lane 16: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (Catalog # PB9966) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
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