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WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35221 CRMP2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35221 CRMP2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35221 CRMP2 antibody. Dilution : 1:3000 Loading : 25microg per lane

CRMP2 antibody

GTX35221
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDPYSL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CRMP2 antibody - KO/KD Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1808
  • Target name
    DPYSL2
  • Target description
    dihydropyrimidinase like 2
  • Target synonyms
    collapsin response mediator protein hCRMP-2; CRMP2; CRMP-2; DHPRP2; dihydropyrimidinase-related protein 2; DRP2; DRP-2; N2A3; ULIP2; ULIP-2; unc-33-like phosphoprotein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16555
  • Protein Name
    Dihydropyrimidinase-related protein 2
  • Scientific Description
    This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimers disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CRMP2/DPYSL2 using anti-CRMP2/DPYSL2 antibody (A02860-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRMP2/DPYSL2 antigen affinity purified polyclonal antibody (Catalog # A02860-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRMP2/DPYSL2 at approximately 65 kDa. The expected band size for CRMP2/DPYSL2 is at 65 kDa.
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