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Lane 1: HepG2 cell lysates; Lane 2: Human heart lysates; Lane 3: Human Liver lysates; Lane 4: Mouse Liver lysates probed with CRY2 Monoclonal Antibody, Unconjugated (bsm-51684M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Human heart lysates; Lane 3: Human Liver lysates; Lane 4: Mouse Liver lysates probed with CRY2 Monoclonal Antibody, Unconjugated (bsm-51684M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Human heart lysates; Lane 3: Human Liver lysates; Lane 4: Mouse Liver lysates probed with CRY2 Monoclonal Antibody, Unconjugated (bsm-51684M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

CRY2 Monoclonal Antibody

BSM-51684M
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetCRY2
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Overview

  • Supplier
    Bioss
  • Product Name
    CRY2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:500-2000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Q3W3
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID1408
  • Target name
    CRY2
  • Target description
    cryptochrome circadian regulator 2
  • Target synonyms
    HCRY2, PHLL2, cryptochrome-2, cryptochrome 2 (photolyase-like), cryptochrome circadian clock 2, growth-inhibiting protein 37
  • Host
    Mouse
  • Protein IDQ49AN0
  • Protein Name
    Cryptochrome-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CRY2 using anti-CRY2 antibody (PB9576). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRY2 antigen affinity purified polyclonal antibody (Catalog # PB9576) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRY2 at approximately 67 kDa. The expected band size for CRY2 is at 67 kDa.
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