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Anti-CRYAB Antibody

CAB13696
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCRYAB
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-CRYAB Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID1410
  • Target name
    CRYAB
  • Target description
    crystallin alpha B
  • Target synonyms
    CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2, MFM2A, MFM2B, alpha-crystallin B chain, epididymis secretory protein Li 101, heat shock protein beta-5, heat shock protein family B member 5, heat-shock 20 kD like-protein, renal carcinoma antigen NY-REN-27, rosenthal fiber component
  • Host
    Rabbit
  • Protein IDP02511
  • Protein Name
    Alpha-crystallin B chain
  • Scientific Description
    CRYAB Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha B Crystallin/CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha B Crystallin/CRYAB2 at approximately 20KD. The expected band size for Alpha B Crystallin/CRYAB is at 20KD.
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