CSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway)
79379
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameCSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway)
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe CSF1R SRE Reporter Kit is designed for monitoring the activity of the CSF1R signaling pathway in cultured cells. The kit contains a transfection-ready vector for CSF1R and SRE luciferase reporter vector. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
- UNSPSC41116133
References
- Cannarile, Michael A., et al. Colony-Stimulating Factor 1 Receptor (CSF1R) Inhibitors in Cancer Therapy. J. Immunother. Cancer, 5(1): 53 (2017)
- Liu, Yang, and Xuetao Cao. The Origin and Function of Tumor-Associated Macrophages. Cellular Molec. Immunology, 12(1): 1-4 (2014)
- Luo, Jian, et al. Colony-Stimulating Factor 1 Receptor (CSF1R) Signaling in Injured Neurons Facilitates Protection and Survival. J. Exp. Med., 210(1): 157-172 (2013)
- Yao, G.-Q., et al. CSF-1 Induces Fos Gene Transcription and Activates the Transcription Factor Elk-1 in Mature Osteoclasts. Calc. Tissue Int., 76(5): 371-378 (2005)