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Western Blot analysis of HuvEc cells using Casein Kinase IIbeta Polyclonal Antibody
Western Blot analysis of HuvEc cells using Casein Kinase IIbeta Polyclonal Antibody
Western Blot analysis of HuvEc cells using Casein Kinase IIbeta Polyclonal Antibody

CSNK2B Antibody

CSB-PA001220
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCSNK2B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CSNK2B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1460
  • Target name
    CSNK2B
  • Target description
    casein kinase 2 beta
  • Target synonyms
    CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS, casein kinase II subunit beta, CK II beta, CSNK2B-LY6G5B-1072, CSNK2B-LY6G5B-1103, CSNK2B-LY6G5B-532, CSNK2B-LY6G5B-560, CSNK2B-LY6G5B-562, Casein kinase II beta subunit, alternative name: G5a, phosvitin, casein kinase 2, beta polypeptide, phosvitin, protein G5a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP67870
  • Protein Name
    Casein kinase II subunit beta
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2B antigen affinity purified polyclonal antibody (Catalog # A03475-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2B at approximately 25 kDa. The expected band size for CSNK2B is at 25 kDa.
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