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ProductsBack/CtBP1 antibody [AT4D6]
WB analysis of the indicated lysates (40ug per lane) using Cystatin B antibody [2F1] at a dilution of 1:500.
WB analysis of the indicated lysates (40ug per lane) using Cystatin B antibody [2F1] at a dilution of 1:500.
WB analysis of the indicated lysates (40ug per lane) using Cystatin B antibody [2F1] at a dilution of 1:500.

CtBP1 antibody [AT4D6]

GTX53708
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetCTBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CtBP1 antibody [AT4D6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT4D6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1487
  • Target name
    CTBP1
  • Target description
    C-terminal binding protein 1
  • Target synonyms
    BARS; brefeldin A-ribosylated substrate; C-terminal-binding protein 1; HADDTS
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13363
  • Protein Name
    C-terminal-binding protein 1
  • Scientific Description
    This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTBP1 using anti-CTBP1 antibody (PB9425). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP1 antigen affinity purified polyclonal antibody (Catalog # PB9425) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP1 at approximately 48 kDa. The expected band size for CTBP1 is at 48 kDa.
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