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Western Blot analysis of HuvEc cells using p120 Polyclonal Antibody
Western Blot analysis of HuvEc cells using p120 Polyclonal Antibody
Western Blot analysis of HuvEc cells using p120 Polyclonal Antibody

CTNND1 Antibody

CSB-PA003612
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCTNND1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CTNND1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1500
  • Target name
    CTNND1
  • Target description
    catenin delta 1
  • Target synonyms
    BCDS2; cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta-1; CTNND; p120; p120 catenin; p120(CAS); p120(CTN); P120CAS; P120CTN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60716
  • Protein Name
    Catenin delta-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of delta 1 Catenin/CAS/CTNND1 using anti-delta 1 Catenin/CAS/CTNND1 antibody (A02333-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: rat RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 antigen affinity purified polyclonal antibody (Catalog # A02333-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for delta 1 Catenin/CAS/CTNND1 at approximately 100-110 kDa. The expected band size for delta 1 Catenin/CAS/CTNND1 is at 108 kDa.
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