CTNS / Cystinosin Antibody

LifeSpan BioSciences

CTNS / Cystinosin Antibody

23

Western blot analysis of Cystinosin expression in Hela (A), HepG2 (B), mouse kidney (C), rat kidney (D) whole cell lysates. Immunofluorescent analysis of Cystinosin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.. ICC, IF (1:50 - 1:200), WB (1:500 - 1:2000) Western blot analysis of Cystinosin expression in Hela (A), HepG2 (B), mouse kidney (C), rat kidney (D) whole cell lysates. Immunofluorescent analysis of Cystinosin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

CTNS

cystinosin, lysosomal cystine transporter

CTNS-LSB, PQLC4, SLC66A4, cystinosin, cystinosis nephropathic

Rabbit

Human

-20°C

12352203