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ProductsBack/CTR1 Polyclonal Antibody, Cy5 Conjugated

CTR1 Polyclonal Antibody, Cy5 Conjugated

BS-10773R-CY5
Bioss
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetSLC31A1
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Overview

  • Supplier
    Bioss
  • Product Name
    CTR1 Polyclonal Antibody, Cy5 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Cy5
  • Gene ID1317
  • Target name
    SLC31A1
  • Target description
    solute carrier family 31 member 1
  • Target synonyms
    COPT1, CTR1, NSCT, high affinity copper uptake protein 1, copper transport 1 homolog, copper transporter 1, solute carrier family 31 (copper transporter), member 1, solute carrier family 31 (copper transporters), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15431
  • Protein Name
    High affinity copper uptake protein 1
  • Reactivity
    Bovine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of SLC31A1/CTR1 using anti-SLC31A1/CTR1 antibody (A03447-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC31A1/CTR1 antigen affinity purified polyclonal antibody (Catalog # A03447-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC31A1/CTR1 at approximately 26KD. The expected band size for SLC31A1/CTR1 is at 21KD.
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