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Western Blot analysis of HepG2 cells using Cathepsin E Polyclonal Antibody
Western Blot analysis of HepG2 cells using Cathepsin E Polyclonal Antibody
Western Blot analysis of HepG2 cells using Cathepsin E Polyclonal Antibody

CTSE Antibody

CSB-PA001331
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCTSE
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Overview

  • Supplier
    Cusabio
  • Product Name
    CTSE Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1510
  • Target name
    CTSE
  • Target description
    cathepsin E
  • Target synonyms
    CATE; cathepsin E; erythrocyte membrane aspartic proteinase; slow-moving proteinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14091
  • Protein Name
    Cathepsin E
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin E antigen affinity purified polyclonal antibody (Catalog # A04874-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin E at approximately 48KD. The expected band size for Cathepsin E is at 43KD.
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