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Ctsg Antibody

CSB-PA006190LA01MO
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityMouse
TargetCtsg
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Overview

  • Supplier
    Cusabio
  • Product Name
    Ctsg Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID13035
  • Target name
    Ctsg
  • Target description
    cathepsin G
  • Target synonyms
    CatG, VSP, cathepsin G, vimentin-specific protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28293
  • Protein Name
    Cathepsin G
  • Scientific Description
    This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9855). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (Catalog # PB9855) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29KD. The expected band size for Cathepsin G is at 29KD.
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