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Western blot analysis of SIRT6 expression in Jurkat cell lysate.
Western blot analysis of SIRT6 expression in Jurkat cell lysate.
Western blot analysis of SIRT6 expression in Jurkat cell lysate.

Anti-SIRT6 Rabbit Monoclonal Antibody

M00611
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
TargetSIRT6
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SIRT6 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HBA-19
  • Gene ID51548
  • Target name
    SIRT6
  • Target description
    sirtuin 6
  • Target synonyms
    SIR2L6, hSIRT6, NAD-dependent protein deacylase sirtuin-6, NAD-dependent protein deacetylase sirtuin-6, SIR2-like protein 6, protein mono-ADP-ribosyltransferase sirtuin-6, regulatory protein SIR2 homolog 6, sir2-related protein type 6, sirtuin type 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6T7
  • Protein Name
    NAD-dependent protein deacylase sirtuin-6
  • Scientific Description
    Boster Bio Anti-SIRT6 Rabbit Monoclonal Antibody catalog # M00611. Tested in WB application. This antibody reacts with Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Miao J, Huang J, Liang Y, et al. Sirtuin 6 is a key contributor to gender differences in acute kidney injury. Cell Death Discov. 2023,9(1):134. doi: 10.1038/s41420-023-01432-y
    Read this paper
  • Xing JJ, Hou JG, Liu Y, et al. Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice. Antioxidants (Basel). 2019,8(9). doi: 10.3390/antiox8090347
    Read this paper

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MSDS

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Figure 1. Western blot analysis of SIRT6 using anti-SIRT6 antibody (A00611-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT6 antigen affinity purified polyclonal antibody (Catalog # A00611-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT6 at approximately 45 kDa. The expected band size for SIRT6 is at 37 kDa.
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