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Goat anti-TPPP/p25

EB09800
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTPPP
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TPPP/p25 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID11076
  • Target name
    TPPP
  • Target description
    tubulin polymerization promoting protein
  • Target synonyms
    25 kDa brain-specific protein; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; p24; p25; p25alpha; p25-alpha; TPPP/p25; TPPP1; tubulin polymerization-promoting protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_008961.1. GeneIDs all Nonhuman: 72948 (mouse); 361466 (rat);. Purification: Antigen affinity purified. Names and symbols: TPPP; tubulin polymerization promoting protein; TPPP/p25; TPPP1; p24; p25; p25alpha; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; tubulin polymerization-promoting protein
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of TPPP using anti-TPPP antibody (A04389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPPP antigen affinity purified polyclonal antibody (Catalog # A04389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPPP at approximately 24 kDa. The expected band size for TPPP is at 24 kDa.
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