MAP2 antibody [HM-2] detects MAP2 protein at by immunofluorescent analysis. Sample: Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
MAP2 antibody [HM-2]
GTX11267
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAvian, Bovine, Chicken, Human, Mouse, Rat
TargetMap2
Overview
- SupplierGeneTex
- Product NameMAP2 antibody [HM-2]
- Delivery Days Customer9
- Antibody SpecificityMonoclonal Anti-MAP2 antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows sele
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHM-2
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID25595
- Target nameMap2
- Target descriptionmicrotubule-associated protein 2
- Target synonymsMAP-2; MAP2R; microtubule-associated protein 2; microtubule-associated protein 2C; Mtap2
- HostMouse
- IsotypeIgG1
- Scientific DescriptionMAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
- ReactivityAvian, Bovine, Chicken, Human, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
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- HIV-1-Tat Protein Inhibits SC35-mediated Tau Exon 10 Inclusion through Up-regulation of DYRK1A Kinase. Kadri F et al., 2015 Dec 25, J Biol ChemRead more
- Astrocytic CCAAT/Enhancer Binding Protein delta Regulates Neuronal Viability and Spatial Learning Ability via miR-135a. Chu YY et al., 2016 Aug, Mol NeurobiolRead more
- Persistent synaptic scaling independent of AMPA receptor subunit composition. Altimimi HF et al., 2013 Jul 17, J NeurosciRead more