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WB analysis of various cell lines using GTX83580 SOX17 antibody [3B10]. Loading : 10 ug per lane Dilution : 1:200
WB analysis of various cell lines using GTX83580 SOX17 antibody [3B10]. Loading : 10 ug per lane Dilution : 1:200
WB analysis of various cell lines using GTX83580 SOX17 antibody [3B10]. Loading : 10 ug per lane Dilution : 1:200

SOX17 antibody [3B10]

GTX83580
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSOX17
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOX17 antibody [3B10] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000. ICC/IF: 1:50-1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64321
  • Target name
    SOX17
  • Target description
    SRY-box transcription factor 17
  • Target synonyms
    PPH7, VUR3, transcription factor SOX-17, SRY (sex determining region Y)-box 17, SRY-box 17, SRY-related HMG-box transcription factor SOX17
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H6I2
  • Protein Name
    Transcription factor SOX-17
  • Scientific Description
    Probable transcriptional activator in the premeiotic germ cells. It binds to the sequences 5-AACAAT-3 or 5-AACAAAG-3.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Generation of a patient-derived induced pluripotent cell line (SCTCi016-A) carrying a homozygous variant in RPE65. Vazquez-Dominguez I et al., 2022 Apr, Stem Cell Res
    Read more
  • Repair of acute liver damage with immune evasive hESC derived hepato-blasts. Liu J et al., 2020 Dec, Stem Cell Res
    Read more

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Figure 1. Western blot analysis of SOX17 using anti-SOX17 antibody (A01665-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX17 antigen affinity purified polyclonal antibody (Catalog # A01665-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX17 at approximately 45 kDa. The expected band size for SOX17 is at 45 kDa.
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