% Signal to Background of Continuous Real-Time TR-FRET XIAP titration (autoubiquitination): Serial dilutions of NEDD4 from 350nM to 43.75nM mixed with UBA1, UBE2D2, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.,Estimated Z-primes of Continuous Real-Time TR-FRET XIAP titration (autoubiquitination): Serial dilutions of XIAP from 350nm to 43.75nM mixed with UBA1, UBE2D2, and TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
XIAP E3 Ligase TR-FRET Kit
SBB-KF0057
Product group Chemicals
Overview
- SupplierSouth Bay Bio
- Product NameXIAP E3 Ligase TR-FRET Kit
- Delivery Days Customer10
- CertificationResearch Use Only
- Hazard InformationNon-hazardous
- Scientific DescriptionSouth Bay Bios XIAP E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto XIAP in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto XIAP in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. - South Bay Bios XIAP E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto XIAP in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto XIAP in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
- Storage Instruction-20°C,-80°C
- UNSPSC12352200