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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA06119A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA06119A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA06119A0Rb at dilution of 1:100

CXCL11 Antibody

CSB-PA06119A0RB
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCXCL11
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Overview

  • Supplier
    Cusabio
  • Product Name
    CXCL11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6373
  • Target name
    CXCL11
  • Target description
    C-X-C motif chemokine ligand 11
  • Target synonyms
    H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1, C-X-C motif chemokine 11, beta-R1, chemokine (C-X-C motif) ligand 11, interferon gamma-inducible protein 9, interferon-inducible T-cell alpha chemoattractant, small inducible cytokine B11, small inducible cytokine subfamily B (Cys-X-Cys), member 11, small inducible cytokine subfamily B (Cys-X-Cys), member 9B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14625
  • Protein Name
    C-X-C motif chemokine 11
  • Scientific Description
    Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCL11 using anti-CXCL11 antibody (M01833). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL11 antigen affinity purified monoclonal antibody (Catalog # M01833) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL11 at approximately 15 kDa. The expected band size for CXCL11 is at 10 kDa.
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