HeLa whole cell extract and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632502/GTX632502_T-44900_20230120_WB_B_23013122_371.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCL16 stained by CXCL16 antibody [GT516] (GTX632502) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632502/GTX632502_44517_20220408_ICC_IF_w_23061202_620.webp "CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCL16 stained by CXCL16 antibody [GT516] (GTX632502) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 antibody [GT516] (GTX632502) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632502/GTX632502_42226_20160118_IHC-P_R_w_23061202_671.webp "CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 antibody [GT516] (GTX632502) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HeLa whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632502/GTX632502_45271_20240126_WB_Fraction_25101920_612.webp "HeLa whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
HeLa whole cell extract and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
CXCL16 antibody [GT516]
GTX632502
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXCL16
Overview
- SupplierGeneTex
- Product NameCXCL16 antibody [GT516]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT516
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID58191
- Target nameCXCL16
- Target descriptionC-X-C motif chemokine ligand 16
- Target synonymsCXCLG16, SR-PSOX, SRPSOX, C-X-C motif chemokine 16, CXC chemokine ligand 16, chemokine (C-X-C motif) ligand 16, scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, small-inducible cytokine B16, transmembrane chemokine CXCL16
- HostMouse
- IsotypeIgG2a
- Protein IDQ9H2A7
- Protein NameC-X-C motif chemokine 16
- Scientific DescriptionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Choi YW, Kim YH, Oh SY, et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh). 2021,8(4):2002497. doi: 10.1002/advs.202002497Read this paper
- Shibata Y, Kobayashi N, Sato T, et al. The clinical significance of CXCL16 in the treatment of advanced non-small cell lung cancer. Thorac Cancer. 2020,11(5):1258-1264. doi: 10.1111/1759-7714.13387Read this paper
Datasheet
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