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CXCL16 Antibody (YA4490, Mouse)

HY-P84793
MedChem Express
TargetCXCL16
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CXCL16 Antibody (YA4490, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID58191
  • Target name
    CXCL16
  • Target description
    C-X-C motif chemokine ligand 16
  • Target synonyms
    CXCLG16, SR-PSOX, SRPSOX, C-X-C motif chemokine 16, CXC chemokine ligand 16, chemokine (C-X-C motif) ligand 16, scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, small-inducible cytokine B16, transmembrane chemokine CXCL16
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    CXCL16 Antibody (YA4490) is a mouse-derived and non-conjugated IgG2a antibody, targeting to CXCL16. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Lane 1: Human A431 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates probed with CXCL16 Polyclonal Antibody, Unconjugated (bs-8871R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB10054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: CEM whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (Catalog # PB10054) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.
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