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ProductsBack/CXCL16 ELISA Pair
ELISA detection of CXCL16 using GTX632390 for capture at a concentration of 5 microg/mL and for detection GTX116706 at a concentration of 1 microg/mL. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was diluted at 1:5,000-1:10,000 and used to detect the primary antibody.
ELISA detection of CXCL16 using GTX632390 for capture at a concentration of 5 microg/mL and for detection GTX116706 at a concentration of 1 microg/mL. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was diluted at 1:5,000-1:10,000 and used to detect the primary antibody.
ELISA detection of CXCL16 using GTX632390 for capture at a concentration of 5 microg/mL and for detection GTX116706 at a concentration of 1 microg/mL. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was diluted at 1:5,000-1:10,000 and used to detect the primary antibody.

CXCL16 ELISA Pair

GTX500036
GeneTex
ApplicationsELISA
DownloadDatasheet
Product group Antibodies
TargetCXCL16
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Overview

  • Supplier
    GeneTex
  • Product Name
    CXCL16 ELISA Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 5microg/mL and the detection antibody at a concentration of 1 microg/ml. Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Gene ID58191
  • Target name
    CXCL16
  • Target description
    C-X-C motif chemokine ligand 16
  • Target synonyms
    chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; C-X-C motif chemokine 16; CXCLG16; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; small-inducible cytokine B16; SRPSOX; SR-PSOX; transmembrane chemokine CXCL16
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB10054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: CEM whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (Catalog # PB10054) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.
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