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Cxcl3 Antibody

CSB-PA09239A0RB
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityMouse
TargetCxcl3
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Overview

  • Supplier
    Cusabio
  • Product Name
    Cxcl3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID330122
  • Target name
    Cxcl3
  • Target description
    C-X-C motif chemokine ligand 3
  • Target synonyms
    Dcip1, Gm1960, C-X-C motif chemokine 3, chemokine (C-X-C motif) ligand 3, dendritic cell inflammatory protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6W5C0
  • Protein Name
    C-X-C motif chemokine 3
  • Scientific Description
    Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRO gamma using anti-GRO gamma antibody (PB9941). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO gamma antigen affinity purified polyclonal antibody (Catalog # PB9941) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRO gamma at approximately 25 kDa. The expected band size for GRO gamma is at 11 kDa.
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