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Formalin-fixed and paraffin embedded human spleen labeled with Anti-CXCL9/MIG Polyclonal Antibody, Unconjugated (bs-2551R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human spleen labeled with Anti-CXCL9/MIG Polyclonal Antibody, Unconjugated (bs-2551R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human spleen labeled with Anti-CXCL9/MIG Polyclonal Antibody, Unconjugated (bs-2551R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

CXCL9 Polyclonal Antibody

BS-2551R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCXCL9
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CXCL9/MIG Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4283
  • Target name
    CXCL9
  • Target description
    C-X-C motif chemokine ligand 9
  • Target synonyms
    chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; gamma-interferon-induced monokine; Humig; MIG; monokine induced by gamma interferon; monokine induced by interferon-gamma; SCYB9; small-inducible cytokine B9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07325
  • Protein Name
    C-X-C motif chemokine 9
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Cordycepin Attenuates IFN-gamma-Induced Macrophage IP-10 and Mig Expressions by Inhibiting STAT1 Activity in CFA-Induced Inflammation Mice Model. Yang R et al., 2020 Apr, Inflammation
    Read more
  • Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats. Wakasa Y et al., 2019 Nov, Mol Med Rep
    Read more
  • Establishment of a chronic obstructive pulmonary disease mouse model based on the elapsed time after LPS intranasal instillation. Lee SY et al., 2018 Mar, Lab Anim Res
    Read more
  • Interleukin (IL)-18, cooperatively with IL-23, induces prominent inflammation and enhances psoriasis-like epidermal hyperplasia. Shimoura N et al., 2017 May, Arch Dermatol Res
    Read more
  • CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment. Zhang QQ et al., 2015 Nov 3, Sci Rep
    Read more
  • Melanoma Induces, and Adenosine Suppresses, CXCR3-Cognate Chemokine Production and T-cell Infiltration of Lungs Bearing Metastatic-like Disease. Clancy-Thompson E et al., 2015 Aug, Cancer Immunol Res
    Read more
  • Splenectomy attenuates murine liver fibrosis with hypersplenism stimulating hepatic accumulation of Ly-6C(lo) macrophages. Yada A et al., 2015 Oct, J Hepatol
    Read more
  • Transcriptomic analysis of hepatic responses to testosterone deficiency in miniature pigs fed a high-cholesterol diet. Cai Z et al., 2015 Feb 6, BMC Genomics
    Read more

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Figure 1. Western blot analysis of CXCL9 using anti-CXCL9 antibody (PB9694). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: A431 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL9 antigen affinity purified polyclonal antibody (Catalog # PB9694) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL9 at approximately 19 kDa. The expected band size for CXCL9 is at 14 kDa.
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