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IHC-P analysis of human breast adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

CYB5R3 antibody [2A10]

GTX84646
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYB5R3
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Overview

  • Supplier
    GeneTex
  • Product Name
    CYB5R3 antibody [2A10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A10
  • Concentration
    0.76 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1727
  • Target name
    CYB5R3
  • Target description
    cytochrome b5 reductase 3
  • Target synonyms
    B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, diaphorase-1, mutant NADH-cytochrome b5 reductase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP00387
  • Protein Name
    NADH-cytochrome b5 reductase 3
  • Scientific Description
    Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ishimura R, El-Gowily AH, Noshiro D, et al. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Nat Commun. 2022,13(1):7857. doi: 10.1038/s41467-022-35501-0
    Read this paper

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Figure 1. Western blot analysis of CYB5R3 using anti-CYB5R3 antibody (A03487-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5R3 antigen affinity purified polyclonal antibody (Catalog # A03487-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5R3 at approximately 34 kDa. The expected band size for CYB5R3 is at 34 kDa.
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