More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3. Positive lysates LY400140 (100ug) and LC400140 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3. Positive lysates LY400140 (100ug) and LC400140 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3. Positive lysates LY400140 (100ug) and LC400140 (20ug) can be purchased separately from OriGene.

CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CF501385
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYB5R3
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E3
  • Gene ID1727
  • Target name
    CYB5R3
  • Target description
    cytochrome b5 reductase 3
  • Target synonyms
    B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, diaphorase-1, mutant NADH-cytochrome b5 reductase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP00387
  • Protein Name
    NADH-cytochrome b5 reductase 3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CYB5R3 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of K562 cells using CYB5R3 Polyclonal Antibody
Product group Antibodies
Cusabio
CYB5R3 AntibodyCSB-PA001856
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCYB5R3
  • SizePrice
Product group Antibodies
Assay Genie
CYB5R3 AntibodyCAB7535
ReactivityHuman
TargetCYB5R3
  • SizePrice
Anti-CYB5R3 Antibody
Product group Antibodies
Antibodies.com
Anti-CYB5R3 AntibodyA83035
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Figure 1. Western blot analysis of CYB5R3 using anti-CYB5R3 antibody (A03487-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5R3 antigen affinity purified polyclonal antibody (Catalog # A03487-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5R3 at approximately 34 kDa. The expected band size for CYB5R3 is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-CYB5R3 Antibody Picoband(r)A03487-4-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCYB5R3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CYB5R3 / B5R AntibodyLS-C831234
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYB5R3
  • SizePrice
Product group Antibodies
MedChem Express
CYB5R3 Antibody (YA2587, Rabbit)HY-P82842
TargetCYB5R3
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-CYB5R3 / Dia 1EB10696
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCYB5R3
  • SizePrice
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.
Product group Antibodies
Atlas Antibodies
Anti-CYB5R3 AntibodyHPA001566
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCYB5R3
  • SizePrice
Product group Antibodies
Biomatik
CYB5R3 Polyclonal AntibodyCAC14005
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCYB5R3
  • SizePrice
WB analysis of HepG2 cell lysate using GTX34344 CYB5R3 antibody.
Product group Antibodies
GeneTex
CYB5R3 antibodyGTX34344
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCYB5R3
  • SizePrice