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ProductsBack/CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI2A10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYB5R3 (Cat# RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3(Cat# TA501353). Positive lysates [LY400140] (100ug) and [LC400140] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYB5R3 (Cat# RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3(Cat# TA501353). Positive lysates [LY400140] (100ug) and [LC400140] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYB5R3 (Cat# RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3(Cat# TA501353). Positive lysates [LY400140] (100ug) and [LC400140] (20ug) can be purchased separately from OriGene.

CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI2A10]

CF501353
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetCYB5R3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI2A10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2A10
  • Gene ID1727
  • Target name
    CYB5R3
  • Target description
    cytochrome b5 reductase 3
  • Target synonyms
    B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, diaphorase-1, mutant NADH-cytochrome b5 reductase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP00387
  • Protein Name
    NADH-cytochrome b5 reductase 3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CYB5R3 mouse monoclonal antibody, clone OTI2A10 (formerly 2A10)
  • Reactivity
    Canine, Human, Monkey
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CYB5R3 using anti-CYB5R3 antibody (A03487-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5R3 antigen affinity purified polyclonal antibody (Catalog # A03487-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5R3 at approximately 34 kDa. The expected band size for CYB5R3 is at 34 kDa.
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