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Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

Cyclin B2 Recombinant Antibody

BSM-52045R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCNB2
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclin B2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID9133
  • Target name
    CCNB2
  • Target description
    cyclin B2
  • Target synonyms
    HsT17299, G2/mitotic-specific cyclin-B2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95067
  • Protein Name
    G2/mitotic-specific cyclin-B2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CCNB2 using anti-CCNB2 antibody (A02040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat testicular tissue lysates, Lane 4: mouse testicular tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB2 antigen affinity purified polyclonal antibody (Catalog # A02040-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB2 at approximately 55KD. The expected band size for CCNB2 is at 45KD.
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