HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature，permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ Cyclooxygenase 2 Antibody（bs-0732R）at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature，permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ Cyclooxygenase 2 Antibody（bs-0732R）at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Cyclooxygenase 2 Polyclonal Antibody
BS-0732R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPtgs2
Overview
- SupplierBioss
- Product NameCyclooxygenase 2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID19225
- Target namePtgs2
- Target descriptionprostaglandin-endoperoxide synthase 2
- Target synonymsCOX2, Cox-2, PES-2, PGHS-2, PHS II, PHS-2, Pghs2, TIS10, gripghs, prostaglandin G/H synthase 2, PGH synthase 2, cyclooxygenase-2, glucocorticoid-regulated inflammatory cyclooxygenase, macrophage activation-associated marker protein P71/73, prostaglandin H2 synthase 2
- HostRabbit
- IsotypeIgG
- Protein IDQ05769
- Protein NameProstaglandin G/H synthase 2
- Storage Instruction-20°C
- UNSPSC12352203
References
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- Zhang LY , Ding L , Shi HH , et al. Eicosapentaenoic acid in the form of phospholipids exerts superior anti-atherosclerosis effects to its triglyceride form in ApoE(-/-) mice. Food Funct. 2019,10(7):4177-4188. doi: 10.1039/c9fo00868cRead this paper
- Wang Y, Yao Y, Zhang C, et al. Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Prostaglandins Other Lipid Mediat. 2019,143:106343. doi: 10.1016/j.prostaglandins.2019.106343Read this paper
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- Zhao X, Zou X, Li Q, et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway. Microb Pathog. 2018,125:431-437. doi: 10.1016/j.micpath.2018.10.008Read this paper
- Chen X, Hou XM, Fan YF, et al. Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis. Mol Med Rep. 2016,14(4):2929-36. doi: 10.3892/mmr.2016.5614Read this paper
- Hu R, Xi L, Cao Q, et al. The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii). Prostaglandins Other Lipid Mediat. 2016,124:9-15. doi: 10.1016/j.prostaglandins.2016.05.006Read this paper
- Li S, Xu X, Jiang M, et al. Lipopolysaccharide induces inflammation and facilitates lung metastasis in a breast cancer model via the prostaglandin E2-EP2 pathway. Mol Med Rep. 2015,11(6):4454-62. doi: 10.3892/mmr.2015.3258Read this paper
- Liu Z, Duan ZJ, Chang JY, et al. Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression. PLoS One. 2014,9(6):e98560. doi: 10.1371/journal.pone.0098560Read this paper
Datasheet
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Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPtgs2
- SizePrice
