HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature，permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ Cyclooxygenase 2 Antibody（bs-0732R）at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature，permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ Cyclooxygenase 2 Antibody（bs-0732R）at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Cyclooxygenase 2 Polyclonal Antibody
BS-0732R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPtgs2
Overview
- SupplierBioss Antibodies
- Product NameCyclooxygenase 2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID19225
- Target namePtgs2
- Target descriptionprostaglandin-endoperoxide synthase 2
- Target synonymsCOX; Cox-; COX2; Cox-2; cyclooxygenase-2; glucocorticoid-regulated inflammatory cyclooxygenase; gripghs; macrophage activation-associated marker protein P71/73; PES-2; Pgh; PGH synthase 2; PGHS; Pghs2; PGHS-2; PHS; PHS II; PHS-2; prostaglandin G/H synthase 2; prostaglandin H2 synthase 2; Tis; TIS10
- HostRabbit
- IsotypeIgG
- Protein IDQ05769
- Protein NameProstaglandin G/H synthase 2
- Storage Instruction-20°C
- UNSPSC12352203
References
- Auricular Electrical Stimulation Alleviates Headache through CGRP/COX-2/TRPV1/TRPA1 Signaling Pathways in a Nitroglycerin-Induced Migraine Rat Model. Liao CC et al., 2019, Evid Based Complement Alternat MedRead more
- LncRNA SNHG1 alleviates IL-1beta-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-kappaB signaling pathways. Lei J et al., 2019 Sep 30, Biosci RepRead more
- Eicosapentaenoic acid in the form of phospholipids exerts superior anti-atherosclerosis effects to its triglyceride form in ApoE-/- mice. Zhang LY et al., 2019 Jul 17, Food FunctRead more
- Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Wang Y et al., 2019 Aug, Prostaglandins Other Lipid MediatRead more
- Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. Zhang J et al., 2019 Jan, Biomed PharmacotherRead more
- Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway. Zhao X et al., 2018 Dec, Microb PathogRead more
- Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis. Chen X et al., 2016 Oct, Mol Med RepRead more
- The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii). Hu R et al., 2016 Jul, Prostaglandins Other Lipid MediatRead more
- Lipopolysaccharide induces inflammation and facilitates lung metastasis in a breast cancer model via the prostaglandin E2-EP2 pathway. Li S et al., 2015 Jun, Mol Med RepRead more
- Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression. Liu Z et al., 2014, PLoS OneRead more
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Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPtgs2
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