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ProductsBack/Cyclophilin A antibody [C1C3]

Cyclophilin A antibody [C1C3]

GTX104698
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPPIA
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclophilin A antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5478
  • Target name
    PPIA
  • Target description
    peptidylprolyl isomerase A
  • Target synonyms
    cyclosporin A-binding protein; CYPA; CYPH; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIase A; rotamase A; T cell cyclophilin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62937
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase A
  • Scientific Description
    This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Relevance of AIF/CypA Lethal Pathway in SH-SY5Y Cells Treated with Staurosporine. Conte M et al., 2021 Dec 27, Int J Mol Sci
    Read more
  • Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer. Shih HJ et al., 2021 Nov 25, Sci Rep
    Read more
  • IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Shih HJ et al., 2020, Am J Cancer Res
    Read more
  • Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells. Chen JF et al., 2020 Aug 5, J Pharm Biomed Anal
    Read more
  • The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex. Rosales Ramirez R et al., 2019 Feb 15, J Virol
    Read more
  • Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells. Lin CY et al., 2016, Cell Death Discov
    Read more
  • Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells. Huang HJ et al., 2014 Dec, Mol Biosyst
    Read more
  • Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance. May EW et al., 2014 Dec, Pharmacol Res
    Read more
  • Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells. Fan CY et al., 2013 Dec, Electrophoresis
    Read more

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Figure 1. Western blot analysis of Cyclophilin A using anti-Cyclophilin A antibody (A01308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin A antigen affinity purified polyclonal antibody (Catalog # A01308) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin A at approximately 185 kDa. The expected band size for Cyclophilin A is at 185 kDa.
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