CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:100. Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min
![CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:685. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636662/GTX636662_T-44529_20220916_ICC_IF_22102723_937.webp "CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:685. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636662/GTX636662_T-44529_20220114_WB_competitor_watermark_w_23061202_889.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636662/GTX636662_44613_20220311_WB_M_R_w_23061202_188.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636662/GTX636662_45257_20231215_WB_TPM_watermark_24061301_112.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:100. Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min
CYLD antibody [HL1260]
GTX636662
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYLD
Overview
- SupplierGeneTex
- Product NameCYLD antibody [HL1260]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1260
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1540
- Target nameCYLD
- Target descriptionCYLD lysine 63 deubiquitinase
- Target synonymsBRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2, ubiquitin carboxyl-terminal hydrolase CYLD, cylindromatosis (turban tumor syndrome), deubiquitinating enzyme CYLD, probable ubiquitin carboxyl-terminal hydrolase CYLD, ubiquitin specific peptidase like 2, ubiquitin thioesterase CYLD, ubiquitin thiolesterase CYLD, ubiquitin-specific-processing protease CYLD
- HostRabbit
- IsotypeIgG
- Protein IDQ9NQC7
- Protein NameUbiquitin carboxyl-terminal hydrolase CYLD
- Scientific DescriptionThis gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1261] (GTX636663) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636663/GTX636663_44648_20220422_WB_w_23061202_589.webp)
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