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CYP1A2 Polyclonal Antibody

BS-25008R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityCanine, Mouse, Rat
TargetCyp1a2
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Overview

  • Supplier
    Bioss
  • Product Name
    CYP1A2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID13077
  • Target name
    Cyp1a2
  • Target description
    cytochrome P450, family 1, subfamily a, polypeptide 2
  • Target synonyms
    CP12, CYPIA2, P450-3, cytochrome P450 1A2, cholesterol 25-hydroxylase, cytochrome P450 family 1 subfamily a polypeptide 1, cytochrome P450, 1a2, aromatic compound inducible, cytochrome P450-P2, cytochrome P450-P3, hydroperoxy icosatetraenoate dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00186
  • Protein Name
    Cytochrome P450 1A2
  • Reactivity
    Canine, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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TargetCyp1a2
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Figure 1. Western blot analysis of Cytochrome P450 1A2/Cyp1a2 using anti-Cytochrome P450 1A2/Cyp1a2 antibody (A00598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome P450 1A2/Cyp1a2 antigen affinity purified polyclonal antibody (Catalog # A00598) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome P450 1A2/Cyp1a2 at approximately 58KD. The expected band size for Cytochrome P450 1A2/Cyp1a2 is at 58KD.
Product group Antibodies
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Anti-Cytochrome P450 1A2/Cyp1a2 Antibody Picoband(r)A00598-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetCyp1a2
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