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Western Blot analysis of Jurkat cells using CYP1B1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CYP1B1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CYP1B1 Polyclonal Antibody

CYP1B1 Antibody

CSB-PA001931
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCYP1B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP1B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1545
  • Target name
    CYP1B1
  • Target description
    cytochrome P450 family 1 subfamily B member 1
  • Target synonyms
    aryl hydrocarbon hydroxylase; ASGD6; CP1B; CYPIB1; cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); dioxin-inducible cytochrome p450; flavoprotein-linked monooxygenase; GLC3A; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P4501B1; xenobiotic monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16678
  • Protein Name
    Cytochrome P450 1B1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP1B1 using anti-CYP1B1 antibody (A00515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: mouse Neuro-2a whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1B1 antigen affinity purified polyclonal antibody (Catalog # A00515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1B1 at approximately 52 kDa. The expected band size for CYP1B1 is at 61 kDa.
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