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Western Blot analysis of HeLa cells using CYP27A1 Polyclonal Antibody
Western Blot analysis of HeLa cells using CYP27A1 Polyclonal Antibody
Western Blot analysis of HeLa cells using CYP27A1 Polyclonal Antibody

CYP27A1 Antibody

CSB-PA001944
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP27A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP27A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1593
  • Target name
    CYP27A1
  • Target description
    cytochrome P450 family 27 subfamily A member 1
  • Target synonyms
    CP27, CTX, CYP27, sterol 26-hydroxylase, mitochondrial, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, cholestanetriol 26-monooxygenase, cytochrome P-450C27/25, cytochrome P450 27, cytochrome P450, family 27, subfamily A, polypeptide 1, cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1, sterol 27-hydroxylase, vitamin D(3) 25-hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02318
  • Protein Name
    Sterol 26-hydroxylase, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. IF analysis of CYP27A1 using anti-CYP27A1 antibody (A02121-2). CYP27A1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5microg/mL rabbit anti-CYP27A1 Antibody (A02121-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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