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Western Blot analysis of Jurkat cells using CYP2A6/7/13 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CYP2A6/7/13 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CYP2A6/7/13 Polyclonal Antibody

CYP2A6/CYP2A7/CYP2A13 Antibody

CSB-PA004739
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCYP2A6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP2A6/CYP2A7/CYP2A13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1548
  • Target name
    CYP2A6
  • Target description
    cytochrome P450 family 2 subfamily A member 6
  • Target synonyms
    1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; CPA6; CYP2A; CYP2A3; CYPIIA6; cytochrome P450 2A6; cytochrome P450 IIA3; cytochrome P450(I); cytochrome P450, family 2, subfamily A, polypeptide 6; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6; flavoprotein-linked monooxygenase; P450C2A; P450PB; xenobiotic monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11509
  • Protein Name
    Cytochrome P450 2A6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP2A6 using anti-CYP2A6 antibody (A00947-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2A6 antigen affinity purified polyclonal antibody (Catalog # A00947-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2A6 at approximately 57KD. The expected band size for CYP2A6 is at 57KD.
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