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Western Blot analysis of HT29 cells using CYP2B6 Polyclonal Antibody
Western Blot analysis of HT29 cells using CYP2B6 Polyclonal Antibody
Western Blot analysis of HT29 cells using CYP2B6 Polyclonal Antibody

CYP2B6 Antibody

CSB-PA001956
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP2B6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP2B6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1555
  • Target name
    CYP2B6
  • Target description
    cytochrome P450 family 2 subfamily B member 6
  • Target synonyms
    1,4-cineole 2-exo-monooxygenase; CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; cytochrome P450 2B6; cytochrome P450 IIB1; cytochrome P450, family 2, subfamily B, polypeptide 6; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6; EFVM; IIB1; P450
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20813
  • Protein Name
    Cytochrome P450 2B6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP2B6 using anti-CYP2B6 antibody (A00861-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2B6 antigen affinity purified polyclonal antibody (Catalog # A00861-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2B6 at approximately 56KD. The expected band size for CYP2B6 is at 56KD.
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