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CYP2C8 Antibody (Internal)

LS-C368574
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCYP2C8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CYP2C8 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1558
  • Target name
    CYP2C8
  • Target description
    cytochrome P450 family 2 subfamily C member 8
  • Target synonyms
    CPC8, CYP2C8DM, CYPIIC8, MP-12/MP-20, cytochrome P450 2C8, P450 form 1, cytochrome P450 IIC2, cytochrome P450 MP-12, cytochrome P450 MP-20, cytochrome P450 form 1, cytochrome P450, family 2, subfamily C, polypeptide 8, cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 8, flavoprotein-linked monooxygenase, microsomal monooxygenase, s-mephenytoin 4-hydroxylase, xenobiotic monooxygenase
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP2C8 using anti-CYP2C8 antibody (A01179-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2C8 antigen affinity purified polyclonal antibody (Catalog # A01179-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2C8 at approximately 50 kDa. The expected band size for CYP2C8 is at 50 kDa.
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