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ProductsBack/CYP3A4 antibody [N2C2], Internal

CYP3A4 antibody [N2C2], Internal

GTX105871
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCYP3A4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CYP3A4 antibody [N2C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.03 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1576
  • Target name
    CYP3A4
  • Target description
    cytochrome P450 family 3 subfamily A member 4
  • Target synonyms
    1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase (sulfoxide-forming); albendazole sulfoxidase; cholesterol 25-hydroxylase; CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; cytochrome P450 3A3; cytochrome P450 3A4; cytochrome P450 HLp; cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450-PCN1; glucocorticoid-inducible P450; HLP; NF-25; nifedipine oxidase; P450C3; P450-III, steroid inducible; P450PCN1; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; VDDR3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08684
  • Protein Name
    Cytochrome P450 3A4
  • Scientific Description
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytochrome P450 3A4/CYP3A4 using anti-Cytochrome P450 3A4/CYP3A4 antibody (A00339-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 antigen affinity purified polyclonal antibody (Catalog # A00339-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome P450 3A4/CYP3A4 at approximately 57 kDa. The expected band size for Cytochrome P450 3A4/CYP3A4 is at 57 kDa.
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WB analysis of various sample lysates using GTX53947 CYP3A4 antibody. Dilution : 1:1000 Loading : 25microg per lane
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