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Western Blot analysis of Hela cells using CYP4F2 Polyclonal Antibody
Western Blot analysis of Hela cells using CYP4F2 Polyclonal Antibody
Western Blot analysis of Hela cells using CYP4F2 Polyclonal Antibody

CYP4F2 Antibody

CSB-PA001980
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP4F2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP4F2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8529
  • Target name
    CYP4F2
  • Target description
    cytochrome P450 family 4 subfamily F member 2
  • Target synonyms
    CPF2, cytochrome P450 4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CYPIVF2, arachidonic acid omega-hydroxylase, cytochrome P450, family 4, subfamily F, polypeptide 2, cytochrome P450, subfamily IVF, polypeptide 2, cytochrome P450-LTB-omega, docosahexaenoic acid omega-hydroxylase, leukotriene-B(4) 20-monooxygenase 1, leukotriene-B(4) omega-hydroxylase 1, phylloquinone omega-hydroxylase CYP4F2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78329
  • Protein Name
    Phylloquinone omega-hydroxylase CYP4F2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP4F2 using anti-CYP4F2 antibody (A01737-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antigen affinity purified polyclonal antibody (A01737-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP4F2 at approximately 50 kDa. The expected band size for CYP4F2 is at 60 kDa.
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