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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CYP51A1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CYP51A1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CYP51A1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47 kDa Observed band size: 57 kDa

CYP51A1 Antibody

CSB-PA614993LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP51A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP51A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1595
  • Target name
    CYP51A1
  • Target description
    cytochrome P450 family 51 subfamily A member 1
  • Target synonyms
    CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1, lanosterol 14-alpha demethylase, CYPLI, cytochrome P450 51A1, cytochrome P450, 51 (lanosterol 14-alpha-demethylase), cytochrome P450, family 51, subfamily A, polypeptide 1, cytochrome P450-14DM, cytochrome P45014DM, cytochrome P450LI, sterol 14-alpha demethylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16850
  • Protein Name
    Lanosterol 14-alpha demethylase
  • Scientific Description
    Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4-dimethyl cholesta-8,14,24-triene-3-beta-ol.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CYP51A1/CYP51 using anti-CYP51A1/CYP51 antibody (A04032-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP51A1/CYP51 antigen affinity purified polyclonal antibody (Catalog # A04032-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP51A1/CYP51 at approximately 57KD. The expected band size for CYP51A1/CYP51 is at 57KD.
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