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CYP7A1 Antibody (clone 3D5)

LS-C812935
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCYP7A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CYP7A1 Antibody (clone 3D5)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D5
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1581
  • Target name
    CYP7A1
  • Target description
    cytochrome P450 family 7 subfamily A member 1
  • Target synonyms
    24-hydroxycholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-hydroxylase; cholesterol 7alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CP7A; CYP7; CYPVII; cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA polypeptide 1
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP7A1 using anti-CYP7A1 antibody (A01601). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP7A1 antigen affinity purified polyclonal antibody (Catalog # A01601) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP7A1 at approximately 58 kDa. The expected band size for CYP7A1 is at 58 kDa.
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