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CYP7A1 Polyclonal Antibody

BS-2399R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYP7A1
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Overview

  • Supplier
    Bioss
  • Product Name
    CYP7A1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1581
  • Target name
    CYP7A1
  • Target description
    cytochrome P450 family 7 subfamily A member 1
  • Target synonyms
    CP7A, CYP7, CYPVII, cytochrome P450 7A1, 24-hydroxycholesterol 7-alpha-hydroxylase, cholesterol 7-alpha-hydroxylase, cholesterol 7-alpha-monooxygenase, cholesterol 7alpha-hydroxylase, cytochrome P450, family 7, subfamily A, polypeptide 1, cytochrome P450, subfamily VIIA polypeptide 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22680
  • Protein Name
    Cytochrome P450 7A1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang N, Yang B, Zhang J, et al. Metabolite profiling of traditional Chinese medicine XIAOPI formula: An integrated strategy based on UPLC-Q-Orbitrap MS combined with network pharmacology analysis. Biomed Pharmacother. 2020,121:109569. doi: 10.1016/j.biopha.2019.109569
    Read this paper
  • Zhang LY , Ding L , Shi HH , et al. Eicosapentaenoic acid in the form of phospholipids exerts superior anti-atherosclerosis effects to its triglyceride form in ApoE(-/-) mice. Food Funct. 2019,10(7):4177-4188. doi: 10.1039/c9fo00868c
    Read this paper
  • Shen X, Chen Y, Zhang J, et al. Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice. Environ Int. 2019,128:146-157. doi: 10.1016/j.envint.2019.04.058
    Read this paper
  • Zhong D, Xie Z, Huang B, et al. Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. Cell Physiol Biochem. 2018,49(3):1163-1179. doi: 10.1159/000493297
    Read this paper
  • Zhang JM, Wang XH, Hao LH, et al. Nrf2 is crucial for the down-regulation of Cyp7a1 induced by arachidonic acid in Hepg2 cells. Environ Toxicol Pharmacol. 2017,52:21-26. doi: 10.1016/j.etap.2017.03.003
    Read this paper
  • Ma Z, Chu L, Liu H, et al. Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats. Sci Rep. 2017,7:44819. doi: 10.1038/srep44819
    Read this paper
  • Guo HL, Hassan HM, Zhang Y, et al. Pyrazinamide Induced Rat Cholestatic Liver Injury through Inhibition of FXR Regulatory Effect on Bile Acid Synthesis and Transport. Toxicol Sci. 2016,152(2):417-28. doi: 10.1093/toxsci/kfw098
    Read this paper
  • Nukaya D, Minami K, Hoshikawa R, et al. Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas. Genes Cells. 2015,20(5):367-81. doi: 10.1111/gtc.12227
    Read this paper
  • Gabbi C, Bertolotti M, Anzivino C, et al. Effects of bile duct ligation and cholic acid treatment on fatty liver in two rat models of non-alcoholic fatty liver disease. Dig Liver Dis. 2012,44(12):1018-26. doi: 10.1016/j.dld.2012.07.001
    Read this paper

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Figure 1. Western blot analysis of CYP7A1 using anti-CYP7A1 antibody (A01601). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP7A1 antigen affinity purified polyclonal antibody (Catalog # A01601) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP7A1 at approximately 58 kDa. The expected band size for CYP7A1 is at 58 kDa.
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