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ICC/IF analysis of PC-12 cells using GTX04545 CYP7B1 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:20
ICC/IF analysis of PC-12 cells using GTX04545 CYP7B1 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:20
ICC/IF analysis of PC-12 cells using GTX04545 CYP7B1 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:20

CYP7B1 antibody

GTX04545
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCYP7B1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CYP7B1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. ICC/IF: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9420
  • Target name
    CYP7B1
  • Target description
    cytochrome P450 family 7 subfamily B member 1
  • Target synonyms
    24-hydroxycholesterol 7-alpha-hydroxylase; 25/26-hydroxycholesterol 7-alpha-hydroxylase; 25-hydroxycholesterol 7-alpha-hydroxylase; 3-hydroxysteroid 7-alpha hydroxylase; CBAS3; CP7B; cytochrome P450 7B1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; oxysterol 7-alpha-hydroxylase; SPG5A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75881
  • Protein Name
    25-hydroxycholesterol 7-alpha-hydroxylase
  • Scientific Description
    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP7B1 using anti-CYP7B1 antibody (A03545-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP7B1 antigen affinity purified polyclonal antibody (A03545-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP7B1 at approximately 58 kDa. The expected band size for CYP7B1 is at 58 kDa.
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