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Western blot analysis of CYP7B1 Antibody (Center) in Hela cell line lysates (35ug/lane). CYP7B1 (arrow) was detected using the purified Pab.
Western blot analysis of CYP7B1 Antibody (Center) in Hela cell line lysates (35ug/lane). CYP7B1 (arrow) was detected using the purified Pab.
Western blot analysis of CYP7B1 Antibody (Center) in Hela cell line lysates (35ug/lane). CYP7B1 (arrow) was detected using the purified Pab.

CYP7B1 Rabbit Polyclonal Antibody

AP17979PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCYP7B1
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    CYP7B1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID9420
  • Target name
    CYP7B1
  • Target description
    cytochrome P450 family 7 subfamily B member 1
  • Target synonyms
    CBAS3, CP7B, SPG5A, cytochrome P450 7B1, 24-hydroxycholesterol 7-alpha-hydroxylase, 25-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1, oxysterol 7-alpha-hydroxylase
  • Host
    Rabbit
  • Protein IDO75881
  • Protein Name
    Cytochrome P450 7B1
  • Scientific Description
    CYP7B1 rabbit polyclonal antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP7B1 using anti-CYP7B1 antibody (A03545-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP7B1 antigen affinity purified polyclonal antibody (A03545-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP7B1 at approximately 58 kDa. The expected band size for CYP7B1 is at 58 kDa.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYP7B1
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Product group Antibodies
RayBiotech
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ReactivityHuman, Mouse, Rat
TargetCYP7B1
  • SizePrice
Product group Antibodies
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ReactivityCanine, Equine, Human, Mouse, Rabbit, Rat
TargetCYP7B1
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IHC image of CSB-PA006461LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
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CYP7B1 AntibodyCSB-PA006461LA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCYP7B1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-CYP7B1 AntibodyEB06161
ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Human
TargetCYP7B1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CYP7B1 Antibody (Internal)LS-C368866
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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TargetCYP7B1
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Immunohistochemical staining of human small intestine shows strong membranous, and cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-CYP7B1 AntibodyHPA017761
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ReactivityHuman
TargetCYP7B1
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IHC-P analysis of human mammary cancer and para-carcinoma tissue using GTX04543 CYP7B1 antibody. Antigen retrieval : Heat mediated citrate buffer Dilution : 1:100
Product group Antibodies
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CYP7B1 antibodyGTX04543
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCYP7B1
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Anti-Cytochrome P450 7B1 AntibodyA96489
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