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Cystatin C antibody

GTX107986
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCst3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cystatin C antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.91 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID13010
  • Target name
    Cst3
  • Target description
    cystatin C
  • Target synonyms
    CysC, cystatin-C, cystatin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21460
  • Protein Name
    Cystatin-C
  • Scientific Description
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
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