
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cytochrome b245 Light Chain Polyclonal Antibody, Unconjugated (bs-3879R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Cytochrome b245 Light Chain Antibody
BS-3879R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYBA
Overview
- SupplierBioss
- Product NameCytochrome b245 Light Chain Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1535
- Target nameCYBA
- Target descriptioncytochrome b-245 alpha chain
- Target synonymsCGD4, p22-PHOX, cytochrome b-245 light chain, cytochrome b light chain, cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245, alpha polypeptide, cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, superoxide-generating NADPH oxidase light chain subunit
- HostRabbit
- IsotypeIgG
- Protein IDP13498
- Protein NameCytochrome b-245 light chain
- Storage Instruction-20°C
- UNSPSC12352203
References
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- Yoshida T, Murayama H, Kawashima M, et al. Apocynin and enzymatically modified isoquercitrin suppress the expression of a NADPH oxidase subunit p22phox in steatosis-related preneoplastic liver foci of rats. Exp Toxicol Pathol. 2017,69(1):9-16. doi: 10.1016/j.etp.2016.10.003Read this paper
- Yue L, Wang W, Wang Y, et al. Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways. Int J Biol Macromol. 2016,89:376-88. doi: 10.1016/j.ijbiomac.2016.05.002Read this paper
- Walton NM, Shin R, Tajinda K, et al. Adult neurogenesis transiently generates oxidative stress. PLoS One. 2012,7(4):e35264. doi: 10.1371/journal.pone.0035264Read this paper






