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ProductsBack/Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI1E12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2. Positive lysates LY424529 (100ug) and LC424529 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2. Positive lysates LY424529 (100ug) and LC424529 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2. Positive lysates LY424529 (100ug) and LC424529 (20ug) can be purchased separately from OriGene.

Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CF501172
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP1A2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI1E12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E12
  • Gene ID1544
  • Target name
    CYP1A2
  • Target description
    cytochrome P450 family 1 subfamily A member 2
  • Target synonyms
    CP12, CYPIA2, P3-450, P450(PA), cytochrome P450 1A2, P450 form 4, aryl hydrocarbon hydroxylase, cholesterol 25-hydroxylase, cytochrome P(3)450, cytochrome P450 4, cytochrome P450, family 1, subfamily A, polypeptide 2, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2, cytochrome P450-P3, dioxin-inducible P3-450, flavoprotein-linked monooxygenase, hydroperoxy icosatetraenoate dehydratase, microsomal monooxygenase, xenobiotic monooxygenase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP05177
  • Protein Name
    Cytochrome P450 1A2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CYP1A2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A2 antigen affinity purified polyclonal antibody (Catalog # PB9545) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1A2 at approximately 58 kDa. The expected band size for CYP1A2 is at 58 kDa.
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