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ProductsBack/Cytochrome P450 2E1 (CYP2E1) Mouse Monoclonal Antibody [Clone ID: OTI5B9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2E1 (Cat# RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1(Cat# TA500734). Positive lysates [LY400264] (100ug) and [LC400264] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2E1 (Cat# RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1(Cat# TA500734). Positive lysates [LY400264] (100ug) and [LC400264] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2E1 (Cat# RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1(Cat# TA500734). Positive lysates [LY400264] (100ug) and [LC400264] (20ug) can be purchased separately from OriGene.

Cytochrome P450 2E1 (CYP2E1) Mouse Monoclonal Antibody [Clone ID: OTI5B9]

CF500734
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP2E1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cytochrome P450 2E1 (CYP2E1) Mouse Monoclonal Antibody [Clone ID: OTI5B9]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5B9
  • Gene ID1571
  • Target name
    CYP2E1
  • Target description
    cytochrome P450 family 2 subfamily E member 1
  • Target synonyms
    CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, cytochrome P450-J, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05181
  • Protein Name
    Cytochrome P450 2E1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CYP2E1 mouse monoclonal antibody, clone OTI5B9 (formerly 5B9)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # A00672) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.
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