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Cytokeratin 18 (KRT18) (KRT18/2808R), CF647 conjugate, 0.1mg/mL [26628-22-8]

Cytokeratin 18 (KRT18) (KRT18/2808R), CF647 conjugate, 0.1mg/mL [26628-22-8]

BNC472808
Biotium
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT18
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Overview

  • Supplier
    Biotium
  • Product Name
    Cytokeratin 18 (KRT18) (KRT18/2808R), CF647 conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    KRT18/2808R
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID3875
  • Target name
    KRT18
  • Target description
    keratin 18
  • Target synonyms
    cell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05783
  • Protein Name
    Keratin, type I cytoskeletal 18
  • Scientific Description
    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 7. IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (A01357-1). Cytokeratin 18 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Cytokeratin 18 Antibody (A01357-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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